Analysis Note

ControlHuman lymph node lysate

Application

Anti-Notch 1 Antibody, extracellular detects level of Notch 1 & has been published & validated for use in WB, IH(P).

Immunohistochemistry Analysis: 1:400 dilution of this antibody has been shown to detect Notch 1 in gastrointestinal and colorectal carcinoma tissue.

Research CategorySignaling

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch-1. notch-2, notch-3, and notch-4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of notch-1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

Immunogen

Epitope: Extracellular domain

GST-tagged recombinant protein corresponding to the extracellular domain of rat Notch 1.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human lymph node lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected Notch 1 on 10 µg of human lymph node lysate.

Specificity

This antibody recognizes Notch 1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~260 kDa observed. Notch 1, extracellular is calculated at ~183 kDa